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Related Experiment Video

Updated: Sep 5, 2025

Quantitative 31P NMR Analysis of Lignins and Tannins
05:57

Quantitative 31P NMR Analysis of Lignins and Tannins

Published on: August 2, 2021

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Advancing quantification methods for polyphenols in brown seaweeds-applying a selective qNMR method compared with the

Marie Emilie Wekre1,2, Sondre Hellesen Brunvoll1, Monica Jordheim1

  • 1Department of Chemistry, University of Bergen, Bergen, Norway.

Phytochemical Analysis : PCA
|July 7, 2022
PubMed
Summary

Quantitative NMR (qNMR) offers a selective method for measuring brown seaweed polyphenols. The Folin-Ciocalteu assay overestimates polyphenols in eulittoral species due to its lack of selectivity.

Keywords:
phlorotanninspolyphenolsqNMRquantificationseaweeds

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Area of Science:

  • Marine Botany
  • Analytical Chemistry
  • Biomass Valorization

Background:

  • Brown seaweeds represent a sustainable biomass resource with significant industrial potential.
  • Polyphenols are key compounds contributing to the value and applications of brown seaweeds.

Purpose of the Study:

  • To quantify total polyphenols in brown seaweeds from various tidal zones.
  • To compare results from a selective quantitative NMR (qNMR) method with the colorimetric Folin-Ciocalteu total phenolic content (TPC) assay.

Main Methods:

  • Quantitative NMR (qNMR) utilized selective peak integration in the aromatic region (7-5.5 ppm).
  • 2D NMR (¹H-¹³C, ¹H-¹⁵N) spectra were used to deselect non-polyphenolic ¹H signals.
  • ¹³C NMR characterization of phlorotannins determined the average protons per aromatic ring for ¹H quantification.

Main Results:

  • qNMR and TPC assays yielded similar polyphenol levels for sublittoral Laminariaceae species.
  • For eulittoral Fucaceae species (Fucus vesiculosus, Ascophyllum nodosum), TPC results were up to three times higher than qNMR.
  • ¹³C NMR indicated the highest phlorotannin polymerization degree in Fucus vesiculosus.

Conclusions:

  • The TPC assay shows comparable polyphenol quantification to selective qNMR for sublittoral brown seaweeds.
  • The TPC assay's lack of selectivity leads to overestimation of polyphenols in eulittoral species compared to qNMR.