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A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
Cellular microRNAs correlate with clinical parameters in multiple injury patients
Diego A Vicente1, Seth A Schobel, Simone Anfossi
1From the Surgical Critical Care Initiative (D.A.V., S.A.S., F.L., V.K., M.J.B., T.G.B., T.A.D., C.J.D., A.D.K., E.A.E.); Department of Surgery (D.A.V., S.A.S., F.L., H.R., V.K., M.J.B., T.A.D., E.A.E.), Uniformed Services University of the Health Sciences; Henry M Jackson Foundation for the Advancement of Military Medicine (S.A.S., F.L., V.K.), Bethesda, Maryland; Translational Molecular Pathology (S.A., M.S., G.A.C.), Houston, Texas; DecisionQ (H.H.), Arlington, Virginia; Department of Surgery (F.L., V.K., M.J.B., E.A.E.), Walter Reed National Military Medical Center, Bethesda, Maryland; Department of Surgery (T.G.B., C.J.D.), Emory University, Atlanta, Georgia; and Department of Surgery (A.D.K.), Duke University, Durham, North Carolina.
MicroRNAs in polytrauma patients correlate with complicated recoveries. Lower microRNA levels and specific markers like miR-21-3p may predict poor outcomes, offering potential therapeutic targets for immunomodulation.
Area of Science:
- Trauma and Critical Care Medicine
- Molecular Biology and Genetics
- Immunology
Background:
- The inflammatory response in polytrauma is complex and linked to injury severity and patient outcomes.
- MicroRNAs (miRNAs) are implicated in modulating immune responses, particularly in infection-mediated pathways.
- The role of miRNA expression in predicting recovery in polytrauma patients remains an area of investigation.
Purpose of the Study:
- To investigate the hypothesis that microRNA expression correlates with complicated recoveries in polytrauma patients (PtPs).
- To identify potential early biomarkers for predicting recovery and prognosis in critically ill trauma patients.
- To explore miRNAs as potential therapeutic targets for immunomodulation in polytrauma.
Main Methods:
- Prospective observational study of 180 polytrauma patients with Injury Severity Score >15.
- Categorization of patients into complicated and uncomplicated recovery groups.
- Measurement of plasma microRNAs and systemic inflammation biomarkers (cytokines, chemokines) at admission (T0) using multiplexed methods.
Main Results:
- Polytrauma patients exhibited lower microRNA levels compared to healthy controls.
- Bivariate analysis showed correlations between microRNA variations and factors including age, comorbidities, complications, and mortality.
- Multivariable analysis identified miR-21-3p and monocyte chemoattractant protein-1 as predictors of complicated recovery (AUC=0.78).
Conclusions:
- Systemic microRNAs are associated with adverse outcomes in polytrauma patients.
- Early detection of microRNA profiles may aid in diagnosing and prognosing complicated recoveries.
- MicroRNAs hold potential as therapeutic targets for modulating immune responses in trauma patients.
