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Phase II Reactions: Methylation Reactions

Phase II Reactions: Methylation Reactions

Methylation is a phase II biotransformation process involving the attachment of a methyl group to a substrate. Enzymes known as methyltransferases orchestrate this reaction.
The mechanism of methylation unfolds in two stages. The first stage sees a methyltransferase enzyme facilitating the transfer of a methyl group from S-adenosylmethionine (SAM) to the substrate, forming S-adenosylhomocysteine (SAH). The second stage involves further metabolism of SAH into homocysteine, which can be recycled...

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Related Experiment Video

Updated: Sep 5, 2025

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity

Published on: March 11, 2012

Author Correction: Making a 6mA demethylase

Sisi Li¹, Jiamu Du²

¹Department of Biochemistry and Molecular Biology, International Cancer Center, Shenzhen University Health Science Center, Shenzhen, China. liss@szu.edu.cn.

Nature Chemical Biology

|July 7, 2022

Summary

No abstract available in PubMed .

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