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A form of human basic fibroblast growth factor with an extended amino terminus
Insights
Researchers determined the amino acid sequence of human placental basic fibroblast growth factor (bFGF), finding it highly homologous to bovine bFGF but with an amino terminal extension.
Area of Science:
- Biochemistry
- Molecular Biology
- Genetics
Background:
- Basic fibroblast growth factor (bFGF) is a key signaling protein involved in various cellular processes.
- Understanding the precise structure of human bFGF is crucial for comprehending its biological functions and potential therapeutic applications.
Purpose of the Study:
- To determine the complete amino acid sequence of human placental bFGF.
- To compare the human sequence with homologous proteins from other species.
Main Methods:
- Combined protein sequencing techniques.
- Utilized complementary DNA (cDNA) sequencing methods.
Main Results:
- The human placental bFGF sequence comprises 157 amino acid residues.
- A calculated molecular weight of 17,464 Da was determined for the human protein.
- High homology was observed between human placental bFGF and bovine pituitary bFGF.
- An amino terminal extension was identified in the human bFGF sequence compared to bovine bFGF and predicted human sequences.
Conclusions:
- The detailed amino acid sequence of human placental bFGF has been elucidated.
- The findings highlight structural similarities and differences between human and bovine bFGF, particularly an N-terminal extension in the human form.
Abstract:
The amino acid sequence of a human placental bFGF was determined by a combination of protein and cDNA sequencing. The placental bFGF consists of 157 amino acid residues with a calculated molecular weight of 17,464 and is highly homologous to bovine pituitary bFGF. The human protein contains an amino terminal extension when compared to the sequence established for bovine bFGF (Esch et al., 1985) and to the sequence of the predicted translation product based on human bFGF cDNA clones (Abraham et al., 1986).