Related Experiment Video
Updated: Sep 5, 2025
06:19
Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
29.5K
Association between Oxidative Burden and Restenosis: A Case-Control Study
Shiva Ganjali1, Atena Mansouri2,3, Mitra Abbasifard4,5
1Department of Medical Biotechnology and Nanotechnology, Mashhad University of Medical Sciences, Mashhad, Iran.
Oxidative Medicine and Cellular Longevity
|July 8, 2022
Summary
In-stent restenosis (ISR) risk can be predicted by measuring oxidative stress markers. Elevated pro-oxidant-antioxidant balance (PAB) and superoxide dismutase (SOD) activity are associated with increased ISR risk.
Area of Science:
- Biochemistry
- Cardiovascular Medicine
- Oxidative Stress Research
Background:
- In-stent restenosis (ISR) is a significant complication following stent implantation, with current treatments like drug-eluting stents and medications offering incomplete protection.
- Identifying patients at high risk for ISR and understanding its biochemical markers are crucial for effective management.
- Oxidative stress (OS) is a key factor implicated in the development and progression of ISR.
Purpose of the Study:
- To investigate the association between various oxidative and anti-oxidative markers and the risk of in-stent restenosis (ISR).
- To identify potential biochemical predictors for ISR risk stratification.
- To explore the role of oxidative burden in the pathophysiology of ISR.
Main Methods:
- A case-control study was conducted with 21 ISR patients, 26 non-ISR patients, and 20 healthy controls.
- Serum levels of malondialdehyde (MDA), thiol groups (GSH), total antioxidant capacity (TAC), glutathione peroxidase (GPx), and superoxide dismutase (SOD) were measured.
- The pro-oxidant-antioxidant balance (PAB) assay was used to assess the overall oxidative burden.
Main Results:
- Malondialdehyde (MDA) levels were significantly higher in the ISR group compared to healthy subjects (P=0.004).
- The pro-oxidant-antioxidant balance (PAB) was significantly elevated in both ISR (P<0.001) and non-ISR (P<0.001) groups versus healthy controls.
- After adjusting for confounders, elevated PAB (OR: 1.866) and SOD activity (OR: 0.0) were significantly associated with ISR risk, while increased TAC (OR: 0.990) was associated with decreased risk.
Conclusions:
- Serum markers of oxidative stress, specifically the pro-oxidant-antioxidant balance (PAB) and superoxide dismutase (SOD) activity, show potential for predicting ISR risk.
- These findings suggest that oxidative stress plays a significant role in the development of ISR.
- Further research into these markers could lead to improved diagnostic and therapeutic strategies for ISR.
More Related Videos
Related Concept Videos
01:23Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches
169
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
169
01:30Coronary Artery Disease I: Introduction
53
Coronary Artery Disease (CAD): An Overview with Scientific InsightsCoronary Artery Disease (CAD), often referred to as C-A-D, is a prevalent blood vessel disorder classified under the broader category of atherosclerosis. Atherosclerosis is a pathological process characterized by the hardening and narrowing of arteries due to the accumulation of atherosclerotic plaques. These plaques are composed of cholesterol, fatty substances, inflammatory cells, calcium, and fibrin, reducing blood flow to...
53