Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Severe infantile obesity with a maternal <i>GNAS</i> deletion and multi-locus imprinting disturbance including hypomethylation of the <i>KCNQ1OT1</i>:TSS-DMR.

Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology·2026
Same author

Efficacy and safety of GH treatment in Japanese pediatric patients with <i>SHOX</i> deficiency: an open-label extension study.

Clinical pediatric endocrinology : case reports and clinical investigations : official journal of the Japanese Society for Pediatric Endocrinology·2026
Same author

Diagnostic work-up in paediatric adrenocortical tumours: an international consensus.

European journal of endocrinology·2026
Same author

GATA4 Single-Amino Acid Deletion in a Male Patient With Congenital Heart Defects, Differences of Sex Development, and Diaphragmatic Hernia.

Clinical genetics·2026
Same author

Genotype-Phenotype Correlations in RPGRIP1-Associated Retinal Dystrophy in a Nationwide Japanese Cohort.

American journal of ophthalmology·2026
Same author

A novel OPHN1 variant associated with cyclic strabismus but in the absence of OPHN1 syndrome.

Scientific reports·2026
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
See all related articles

Related Experiment Video

Updated: Sep 5, 2025

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring
06:11

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

756

Efficient Superovulation and Egg Collection from Mice.

Miyuki Shindo1, Kenji Miyado2, Woojin Kang2

  • 1Division of Laboratory Animal Resources, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya, Tokyo 157-8535, Japan.

Bio-Protocol
|July 8, 2022
PubMed
Summary
This summary is machine-generated.

Superovulation protocols enhance egg yield in mice, reducing experimental animal numbers. A novel method using anti-inhibin serum plus equine chorionic gonadotropin (eCG) proves more efficient than conventional treatments for obtaining high-quality eggs.

Keywords:
Anti-inhibin serumChorionic GonadotropinCumulus cellsEggHyaluronidaseMouseOocyteSuperovulation

More Related Videos

Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
05:36

Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

8.5K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

9.9K

Related Experiment Videos

Last Updated: Sep 5, 2025

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring
06:11

Author Spotlight: Exploring the Long-Term Health Impacts of Intracytoplasmic Sperm Injection on Offspring

Published on: May 17, 2024

756
Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination
05:36

Author Spotlight: Inducing Pseudopregnancy in Female Mice Without the Need for Vasectomized Males Prior to Non-Surgical Embryo Transfer or Artificial Insemination

Published on: July 7, 2023

8.5K
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
06:46

Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice

Published on: April 2, 2020

9.9K

Area of Science:

  • Reproductive Biology
  • Animal Models
  • Developmental Biology

Background:

  • Superovulation increases egg yield in female mice, minimizing the number of animals required for research.
  • Conventional superovulation uses equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG), yielding approximately 20 eggs per mouse, double the spontaneous ovulation rate.
  • C57BL/6 mice are commonly used, with spontaneous ovulation yielding about 10 eggs.

Purpose of the Study:

  • To describe and compare conventional and non-conventional superovulation methods in mice.
  • To detail egg collection procedures following superovulation.
  • To highlight the suitability of collected eggs for downstream applications like intracytoplasmic sperm injection and cryopreservation.

Main Methods:

  • Intraperitoneal injection of superovulation reagents.
  • Comparison of conventional (eCG + hCG) and non-conventional (anti-inhibin serum [AIS] + eCG) stimulation protocols.
  • Egg collection post-ovulation and removal of cumulus cells using hyaluronidase.

Main Results:

  • The non-conventional method combining AIS and eCG demonstrated higher efficiency than the conventional method.
  • Optimized injection-to-sampling intervals yield a large number of high-quality eggs.
  • Collected eggs, after cumulus cell removal, are suitable for advanced reproductive techniques.

Conclusions:

  • Non-conventional superovulation using AIS + eCG offers an improved approach for maximizing egg retrieval in mice.
  • This protocol facilitates efficient egg collection for various research applications, including genetic engineering studies.
  • The described methods support the ethical reduction of animal use in scientific experiments.