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Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies
Published on: November 10, 2023
Alterations in bone marrow microRNA expression profiles on infection with avian pathogenic Escherichia coli
Lei Yin1, Xuehuai Shen1, Danjun Zhang1
1Livestock and Poultry Epidemic Diseases Research Center of Anhui Province, Institute of Animal Husbandry and Veterinary Science, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China; Anhui Province Key Laboratory of Livestock and Poultry Product Safety Engineering, Hefei, Anhui 230031, China.
Abstract:
Avian pathogenic Escherichia coli (APEC) is one of the most common avian bacterial diseases globally. The bone marrow is a reservoir of immature immune cells. To elucidate the role of bone marrow microRNAs (miRNAs) in regulating the host response to APEC infection, we performed miRNA-seq to investigate alterations in the expression of bone marrow miRNAs in three groups of specific pathogen-free chickens: non-challenged (NC) and challenged with APEC for 12 h (C12) and 24 h (C24). Twenty and 19 differentially expressed miRNAs (fold change >2, P < 0.01) were identified on comparing the NC and C12 and the NC and C24 groups, respectively. On functional annotation analysis of target genes of differentially expressed miRNAs, we found that the gene ontology term "immune system process" was significantly enriched at both 12 h and 24 h; moreover, several important signaling pathways were triggered in response to APEC infection, such as MAPK, cGMP-PKG, Notch, and cAMP pathways. In addition, we performed reverse transcription quantitative real-time PCR (qRT-PCR) to validate the differential expression of miRNAs. qRT-PCR data were similar to the sequencing data. On constructing an miRNA-target gene network, gga-miR-2127, gga-miR-6643-5p, and gga-miR-6567-3p were found to potentially play a vital role in the immune process. Overall, our findings provide deeper insights into miRNA transcriptome changes involved in the immune response of the chicken bone marrow to APEC infection.
Insights
Avian pathogenic Escherichia coli (APEC) infection alters bone marrow microRNA (miRNA) expression in chickens. These changes impact immune responses and involve key signaling pathways, offering insights into host defense mechanisms.
Area of Science:
- * Immunology
- * Molecular Biology
- * Avian Pathology
Background:
- * Avian pathogenic Escherichia coli (APEC) is a significant cause of disease in poultry worldwide.
- * The bone marrow serves as a critical reservoir for immune cells, playing a key role in host defense.
- * MicroRNAs (miRNAs) are known regulators of gene expression and immune responses.
Purpose of the Study:
- * To investigate the role of bone marrow miRNAs in the host response to APEC infection in chickens.
- * To identify differentially expressed miRNAs and their target genes in the bone marrow following APEC challenge.
- * To elucidate the molecular pathways regulated by miRNAs during APEC infection.
Main Methods:
- * miRNA sequencing (miRNA-seq) was performed on bone marrow samples from non-challenged and APEC-challenged chickens at 12 and 24 hours post-infection.
- * Differential expression analysis was conducted to identify significant miRNA changes.
- * Functional annotation, pathway analysis, and miRNA-target gene network construction were employed.
Main Results:
- * Twenty and nineteen differentially expressed miRNAs were identified at 12h and 24h post-infection, respectively.
- * Functional analysis revealed enrichment of the
- immune system process
- gene ontology term.
- * Key signaling pathways including MAPK, cGMP-PKG, Notch, and cAMP were activated. Specific miRNAs like gga-miR-2127, gga-miR-6643-5p, and gga-miR-6567-3p were implicated in immune processes.
Conclusions:
- * APEC infection induces significant alterations in the bone marrow miRNA transcriptome in chickens.
- * These miRNA changes are associated with the regulation of immune system processes and specific signaling pathways.
- * The study provides novel insights into the molecular mechanisms underlying chicken bone marrow immunity against APEC infection.
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