Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fusion of Secretory Vesicles with the Plasma Membrane

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...

Overview of Secretory Vesicles

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Hypothalamic POMC neurons regulate intestinal glucose absorption via a gut-brain circuit.

Nature communications·2026

Same author

Repetitive transcranial magnetic stimulation suppresses glia-associated neuroinflammation and promotes peripheral nerve recovery in neuropathic pain.

Frontiers in immunology·2026

Same author

Precision Engineering of Extracellular Vesicles as Programmable Carriers for mRNA Therapeutics.

International journal of nanomedicine·2026

Same author

Exposure to Nanoplastics Disrupts Neurotransmitter Release in Rat Hippocampal Neurons.

Environment & health (Washington, D.C.)·2026

Same author

Direct Observation of Nanometer-Sized Steps of Single Myosin VI Molecules in Living Cells.

Nano letters·2026

Same author

Initial Distance-Dependent Mean Force Drives Synaptic Vesicle Motion Toward Fusion Sites in Stimulated Hippocampal Neurons.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025

Search research articles

Related Experiment Video

Updated: Sep 5, 2025

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Published on: February 7, 2025

Single vesicle tracking for studying synaptic vesicle dynamics in small central synapses.

Chungwon Park¹, Sangyong Jung², Hyokeun Park³

¹Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, 999077, Hong Kong.

Current Opinion in Neurobiology

|July 8, 2022

Summary

Tracking single synaptic vesicles reveals crucial dynamics in neurons. This technique enhances understanding of neurotransmission and neurodegenerative diseases.

Keywords:

Neurodegenerative disease Single particle tracking Synaptic transmission Synaptic vesicles Vesicle motion Vesicle pool

More Related Videos

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Published on: November 11, 2011

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

Related Experiment Videos

Last Updated: Sep 5, 2025

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

Published on: February 7, 2025

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Quantitative Analysis of Synaptic Vesicle Pool Replenishment in Cultured Cerebellar Granule Neurons using FM Dyes

Published on: November 11, 2011

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

Area of Science:

Neuroscience
Cell Biology
Biophysics

Background:

Synaptic transmission relies on a steady supply and regulated movement of synaptic vesicles.
Understanding synaptic vesicle dynamics is crucial for comprehending neuronal function but has been limited by technical challenges.

Purpose of the Study:

To review recent advancements in single synaptic vesicle tracking.
To highlight how these advancements illuminate synaptic vesicle dynamics in central synapses.
To discuss the implications for synaptic transmission and neurodegenerative disease mechanisms.

Main Methods:

Utilizing advanced fluorescence imaging techniques for high spatiotemporal resolution tracking of single synaptic vesicles.
Observing vesicle dynamics within and outside presynaptic terminals in living neurons.
Analyzing the influence of vesicle location, recycling stage, and neuronal activity on dynamics.

Main Results:

Single vesicle tracking is a powerful tool for studying synaptic vesicle dynamics.
Revealed location-dependent and activity-dependent vesicle dynamics.
Demonstrated the influence of vesicle recycling stages on their movement.

Conclusions:

Single synaptic vesicle tracking provides unprecedented insights into synaptic vesicle dynamics.
These dynamics are critical for normal synaptic transmission.
Dysregulation of synaptic vesicle dynamics may contribute to neurodegenerative diseases.