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Updated: Sep 5, 2025

Supercritical Nitrogen Processing for the Purification of Reactive Porous Materials
Published on: May 15, 2015
Framework to improve biohydrogen generation with estrogen co-metabolism under complete suppression of nitrogen source
Zainab Syed1, Monika Sogani1, Gopesh Sharma1
1Department of Biosciences, Manipal University Jaipur, Jaipur 303007, Rajasthan, India.
Abstract:
The current work provides insights for improving the hydrogen output while degrading emerging contaminants using Rhodopseudomonas palustris. The changes in the growth rate of a microorganism due to different substrate inputs affects the hydrogen production due to metabolic route changes. The different ratios of glutamate and glycerol as nitrogen and carbon sources along with the presence of ethinylestradiol (EE2) in the photofermenter affected the flux of electrons being directed towards biosynthesis and biohydrogen generation. The combination of glutamate and glycerol in different ratios (Glu:Gly; 0, 0.20 and 0.54) along with estrogen showed no significant difference in the bacteriochlorophyll concentrations. The highest biomass concentration (0.013 h-1) was in ratio of 0.54 while maximum specific hydrogen production (1.9 ± 0.05 ml g-1 biomass h-1) was observed under complete suppression of nitrogen (0; without Glu; non-growing condition) with resultant improved estrogen degradation of about 78% in 168 h by R. palustris strain MDOC01.
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