Related Experiment Video
Updated: Sep 5, 2025
08:43
Loss- and Gain-of-function Approach to Investigate Early Cell Fate Determinants in Preimplantation Mouse Embryos
Published on: June 6, 2016
8.9K
cd26 Knockdown Negatively Affects Porcine Parthenogenetic Preimplantation Embryo Development
In-Sul Hwang1,2, Joohyun Shim3, Keon Bong Oh1
1Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Jeonbuk, Korea.
Animals : an Open Access Journal From MDPI
|July 9, 2022
Summary
CD26 is crucial for porcine embryonic development, as its downregulation halts blastocyst formation and increases apoptosis. This protein is essential for successful parthenogenetic activation in pig embryos.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Molecular Biology
Background:
- CD26 is widely distributed in human tissues and marks embryo implantation.
- Its role in porcine pre-implantation embryo development remains largely uncharacterized.
Purpose of the Study:
- To investigate the function of CD26 in porcine pre-implantation embryonic development.
- To determine if CD26 regulates porcine embryonic development through siRNA-induced downregulation.
Main Methods:
- Microinjection of CD26 siRNA into MII oocytes.
- Electrical parthenogenetic activation in a Mannitol medium.
- Transcriptome resequencing to analyze gene expression changes.
Main Results:
- CD26 inhibition did not affect oocyte cleavage but arrested development at the blastocyst stage.
- CD26-depleted blastocysts exhibited significantly increased apoptosis.
- Transcriptome analysis revealed differential expression of 38 genes related to apoptosis, reactive oxygen species, and ribosomal proteins.
Conclusions:
- CD26 is essential for proper porcine parthenogenetic activation and embryonic development.
- Downregulation of CD26 negatively impacts blastocyst formation and increases cellular apoptosis.
- CD26 influences key developmental pathways, including apoptosis and oxidative stress response.
