Paper Biosensor for the Detection of NT-proBNP Using Silver Nanodisks as Electrochemical Labels

Area of Science:

• Nanotechnology
• Electrochemistry
• Biomarker Detection

Background:

• Heart failure diagnosis relies on biomarkers like NT-proBNP.
• Current detection methods require optimization for sensitivity and dynamic range.
• Silver nanoparticles offer potential as electrochemical labels due to their unique properties.

Purpose of the Study:

• To investigate silver nanodisks (AgNDs) as electrochemical labels for NT-proBNP detection.
• To evaluate the performance of AgNDs compared to other silver nanoparticle shapes.
• To establish the dynamic range of the AgND-based assay for heart failure risk stratification.

Main Methods:

• Synthesis of silver nanodisks (AgNDs) with specific dimensions.
• Development of a metalloimmunoassay using AgNDs as electrochemical labels.
• Detection via electrochemically activated galvanic exchange (GE) and anodic stripping voltammetry (ASV).

Main Results:

• AgNDs demonstrated a superior dynamic range compared to Ag nanocubes and nanospheres.
• The model assay showed a linear dynamic range of 1.5–30.0 pM AgNDs.
• The NT-proBNP assay achieved a dynamic range of 0.03–4.0 nM, overlapping the clinical risk stratification range.

Conclusions:

• Silver nanodisks are effective electrochemical labels for NT-proBNP detection.
• The unique GE mechanism of AgNDs contributes to improved assay performance.
• This method shows promise for sensitive and accurate heart failure risk stratification.