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Updated: Sep 5, 2025
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
Published on: April 21, 2023
Characterization and Antibacterial Evaluation of Biodegradable Mannose-Conjugated Fe-MIL-88NH2 Composites Containing
Haseena1, Muddaser Shah2,3, Khadija Rehman4
1Institute of Chemical Sciences, University of Peshawar, Peshawar 25120, Pakistan.
Abstract:
The emergence of bacterial resistance has increased the economic burden of infectious diseases dramatically during the previous few decades. Multidrug resistance (MDR) is difficult to cure in both Gram-negative and positive bacteria and is often incurable with traditional and broad-range antibiotics. Therefore, developing techniques to increase the antibacterial activity of therapeutic drugs is essential. Metal-organic frameworks (MOFs) are extremely versatile hybrid materials made of metal ions coupled via organic bridging ligands. They have been widely used as an excellent vehicle for drug delivery due to their low toxicity, biodegradability, and structural stability upon loading and functionalization. The present study focused on the synthesis of mannose (MNS)-coated MOFs with enhanced surface contact with S. aureus cells. The MNS coating on the surface of MOFs enhances their adherence to bacteria by binding to lectins present on the bacterial cell, resulting in improved VCM cellular penetration and activity against resistant bacteria. Various techniques, including atomic force microscopy, DLS, TGA, FT-IR, and DSC, were employed to analyze MNS-coated MOFs. They were also evaluated for their efficacy against resistant S. aureus. The results indicated that when VCM was loaded into MNS-coated MOFs, their bactericidal activity rose dramatically, resulting in the greater suppression of resistant S. aureus. AFM investigation of S. aureus strains demonstrated total morphological distortion after treatment with MNS-coated drug-loaded MOFs. The results of this work suggest that MNS-coated MOFs may be effective for reversing bacterial resistance to VCM and open new pathways for improving antibiotic therapy for diseases associated with MDR.
Insights
Mannose-coated metal-organic frameworks (MOFs) enhance vancomycin (VCM) delivery to combat drug-resistant bacteria. This novel approach significantly boosts VCM efficacy against resistant Staphylococcus aureus, offering new therapeutic strategies.
Area of Science:
- Materials Science
- Nanotechnology
- Microbiology
Background:
- Bacterial resistance to antibiotics, particularly multidrug resistance (MDR), presents a significant global health and economic challenge.
- Traditional antibiotics are often ineffective against resistant Gram-negative and Gram-positive bacteria, necessitating innovative therapeutic strategies.
- Metal-organic frameworks (MOFs) offer a promising platform for drug delivery due to their tunable properties, low toxicity, and structural stability.
Purpose of the Study:
- To synthesize mannose (MNS)-coated MOFs for enhanced delivery of vancomycin (VCM) to combat resistant bacteria.
- To investigate the adherence and cellular penetration of MNS-coated MOFs to Staphylococcus aureus (S. aureus) cells.
- To evaluate the efficacy of VCM-loaded MNS-coated MOFs against VCM-resistant S. aureus.
Main Methods:
- Synthesis of MNS-coated MOFs.
- Characterization using atomic force microscopy (AFM), dynamic light scattering (DLS), thermogravimetric analysis (TGA), Fourier-transform infrared spectroscopy (FT-IR), and differential scanning calorimetry (DSC).
- Evaluation of bactericidal activity against resistant S. aureus strains and morphological analysis using AFM.
Main Results:
- MNS coating enhanced MOF adherence to S. aureus by interacting with bacterial lectins.
- VCM-loaded MNS-coated MOFs demonstrated significantly increased bactericidal activity against resistant S. aureus.
- AFM revealed complete morphological distortion of S. aureus cells after treatment with MNS-coated drug-loaded MOFs.
Conclusions:
- MNS-coated MOFs are effective in reversing bacterial resistance to VCM.
- This approach enhances VCM cellular penetration and efficacy against resistant S. aureus.
- MNS-coated MOFs represent a promising strategy for developing improved antibiotic therapies for MDR infections.
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