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Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Testing Targeted Therapies in Cancer using Structural DNA Alteration Analysis and Patient-Derived Xenografts

Published on: July 25, 2020

Molecular testing of soft tissue tumors.

Douglas Rottmann¹, Eman Abdulfatah¹, Liron Pantanowitz¹

¹Department of Pathology, Michigan Medicine, University of Michigan, Ann Arbor, Michigan, USA.

Diagnostic Cytopathology

|July 9, 2022

Summary

Diagnosing soft tissue tumors, especially with limited samples, often requires molecular testing. This review explores various molecular techniques for accurate tumor classification and prognostication.

Keywords:

cytopathology fluorescent in situ hybridization molecular next-generation sequencing polymerase chain reaction sarcoma soft tissue

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Published on: March 21, 2018

Area of Science:

Pathology
Oncology
Molecular Diagnostics

Background:

Soft tissue tumor diagnosis is challenging with limited biopsy material, necessitating ancillary testing.
Molecular testing is increasingly vital for classifying and predicting outcomes of soft tissue tumors.
Newer entities like CIC-rearranged sarcoma require specific molecular diagnostic approaches.

Approach:

This review examines diverse molecular tests, including in situ hybridization, PCR, and next-generation sequencing.
It also considers immunohistochemical proxies as a recent advancement in molecular testing.
The focus is on characterizing tumors from small biopsy and cytologic samples.

Key Points:

Molecular tests are crucial for accurate soft tissue tumor diagnosis and classification.
Various molecular techniques offer different advantages for analyzing limited tissue samples.
Immunohistochemistry can serve as a proxy for molecular testing in specific cases.

Conclusions:

The review evaluates the utility of different molecular tests for soft tissue tumors.
It assesses their role in characterizing tumors obtained via small biopsies and cytology.
Understanding these tests aids in precise tumor characterization and patient management.