Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Flow Cytometric Screening Reveals Nucleic Acid Dyes Aberrantly Stain Peptidoglycan Sacculi.

ACS infectious diseases·2026
Same author

Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.

Nature chemical biology·2026
Same author

A system for in vitro selection of fully 2'-modified RNA aptamers.

Organic & biomolecular chemistry·2025
Same author

Discovering microbiota functions via chemical probe incorporation for targeted sequencing.

Current opinion in chemical biology·2024
Same author

O-GlcNAcylation of enolase 1 serves as a dual regulator of aerobic glycolysis and immune evasion in colorectal cancer.

Proceedings of the National Academy of Sciences of the United States of America·2024
Same author

Chemoenzymatic Labeling, Detection and Profiling of Core Fucosylation in Live Cells.

Journal of the American Chemical Society·2024
Same journal

A viral ORFeome library for systems-level genetic dissection of host-pathogen interactions.

Cell·2026
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
See all related articles

Related Experiment Video

Updated: Sep 5, 2025

Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

266

Tools for mammalian glycoscience research.

Matthew E Griffin1, Linda C Hsieh-Wilson2

  • 1Department of Chemistry, University of California, Irvine, Irvine, CA 92697, USA; Department of Microbiology and Immunology, Scripps Research, La Jolla, CA 92037, USA.

Cell
|July 9, 2022
PubMed
Summary
This summary is machine-generated.

Cellular glycans are vital for biological functions. This primer explores advanced methods for identifying, monitoring, and manipulating these complex carbohydrates in mammals.

Keywords:
carbohydratesglycansglycobiologymammalian biology

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.3K
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

7.0K

Related Experiment Videos

Last Updated: Sep 5, 2025

Mitochondrial Preparation from Microglia for Glycan Analysis
06:40

Mitochondrial Preparation from Microglia for Glycan Analysis

Published on: May 30, 2025

266
Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments
10:59

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

1.3K
Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

7.0K

Area of Science:

  • Glycobiology
  • Mammalian cell biology
  • Biochemistry

Background:

  • Cellular carbohydrates, also known as glycans, play crucial roles in diverse biological processes.
  • The structural complexity and functional variety of glycans offer significant potential for biological discovery.
  • Understanding glycan roles is essential across many biological disciplines.

Purpose of the Study:

  • To provide an overview of essential techniques for studying glycans.
  • To highlight recent technological advancements in glycan analysis and manipulation.
  • To serve as a primer for researchers interested in mammalian glycobiology.

Main Methods:

  • Review of established and emerging methodologies for glycan identification.
  • Discussion of techniques for monitoring glycan dynamics in living systems.
  • Exploration of methods for targeted manipulation of cellular glycans.

Main Results:

  • Identification of key methods for comprehensive glycan profiling.
  • Highlighting breakthrough technologies enabling real-time glycan observation.
  • Summarizing strategies for functional glycan perturbation in mammalian cells.

Conclusions:

  • Advanced techniques are crucial for unlocking the full potential of glycan research.
  • The described methods facilitate deeper understanding of glycan-mediated biological functions.
  • This primer equips researchers with knowledge of cutting-edge glycan technologies.