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Updated: Sep 5, 2025

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Single mutations toggle the substrate selectivity of multifunctional Camptotheca secologanic acid synthases

Justin C Miller1, Mary A Schuler2

  • 1Department of Chemistry, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.

The Journal of Biological Chemistry
|July 9, 2022
PubMed
Summary

Researchers identified key amino acid residues in terpene indole alkaloid (TIA) pathways that control enzyme selectivity. These findings enable tailored production of medicinal compounds like TIAs.

Keywords:
Camptotheca acuminataancestral sequence reconstructioncytochrome P450enzyme catalysisenzyme mutationhomology modelingnatural product biosynthesisplant biochemistrysecondary metabolism

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