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Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
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Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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pH modulation in adhesive cells with a protonic biotransducer.

Mingyin Cui1, Momoka Takahashi2, Yukun Chen1

  • 1Graduate School of Information, Production and Systems, Waseda University, Kitakyushu, Fukuoka 808-0135, Japan.

Bioelectrochemistry (Amsterdam, Netherlands)
|July 10, 2022
PubMed
Summary

This study introduces a novel sulfonated polyaniline (SPA) biotransducer, enhanced with carbon nanotubes (CNTs), for precisely controlling intracellular pH. This protonic device enables targeted modulation of cellular functions by manipulating proton (H+) signals.

Keywords:
BiotransducerCell functionProton signalpH modulation

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Area of Science:

  • Biomedical Engineering
  • Cellular Physiology
  • Materials Science

Background:

  • Protons (H+) are critical for cellular and organelle functions.
  • Precise control of intracellular pH is essential for regulating physiological processes.
  • Existing biotransducers lack the specificity for effective intracellular pH modulation.

Purpose of the Study:

  • To develop and demonstrate a novel sulfonated polyaniline (SPA) biotransducer for modulating intracellular pH.
  • To enhance the SPA biotransducer's performance using a carbon nanotube (CNT) supportive layer.
  • To investigate the biotransducer's capacity to influence cellular functions via proton (H+) signaling.

Main Methods:

  • Fabrication of a hybrid SPA-CNT biotransducer electrode.
  • Application of a low potential (< ±0.6 V) to modulate intracellular pH in C6 cells.
  • Comparison of SPA-CNT biotransducer performance with a poly(3,4-ethylenedioxythiophene) (PEDOT)-based transducer.
  • Measurement of intracellular pH, plasma membrane potential, and mitochondrial functions.

Main Results:

  • The SPA-CNT biotransducer successfully modulated intracellular pH with high H+ selectivity and capacity.
  • A potential difference of less than ±0.6 V was sufficient for effective pH modulation.
  • The biotransducer influenced plasma membrane potential, molecular flow, and mitochondrial functions (mitochondria membrane potential, reactive oxygen species, intracellular Ca2+).
  • A non-selective PEDOT-based biotransducer failed to achieve significant pH modulation.

Conclusions:

  • The developed protonic biotransducer offers a new method for engineering cellular processes via direct H+ signal transfer.
  • The SPA-CNT hybrid electrode demonstrates superior performance for selective and efficient intracellular pH control.
  • This technology provides a platform for precise manipulation of cellular functions by controlling proton gradients.