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Related Experiment Video

Updated: Sep 5, 2025

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Published on: June 4, 2014

Immunohistochemical analysis of the developing mouse cortex.

Mohamad-Reza Aghanoori¹, Kaylan M L Burns², Maneesha Subha²

¹Department of Medical Genetics, Cumming School of Medicine, Calgary, AB, Canada; Alberta Children's Hospital Research Institute, Calgary, AB, Canada.

Methods in Cell Biology

|July 10, 2022

Summary

This summary is machine-generated.

This study details a mouse brain immunohistochemistry protocol to analyze neural stem/precursor cell (NPC) development. The method aids in understanding cortical development and neurodevelopmental disorders.

Keywords:

Cerebral cortex Development Immunohistochemistry Mammalian brain Neural stem/precursor cells

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Area of Science:

Neuroscience
Developmental Biology
Cell Biology

Background:

Mammalian brain development, particularly the cerebral cortex, relies on precise neural stem/precursor cell (NPC) proliferation and differentiation.
Disruptions in NPC homeostasis are linked to abnormal cortical development and neurodevelopmental disorders.

Purpose of the Study:

To provide a detailed protocol for immunohistochemistry on mouse cortical tissues.
To enable the study of neural stem/precursor cell populations and their role in cortical development.

Main Methods:

Preparation of mouse cortical tissues.
Step-by-step immunohistochemistry protocol.
Utilizing molecular markers and thymidine analog methods for NPC analysis.

Main Results:

The described protocol allows for the analysis of neural stem/precursor cell populations in developing mouse cortex.
The methodology can be adapted by optimizing assay conditions for specific experimental needs.

Conclusions:

This immunohistochemistry technique is valuable for investigating the mechanisms of cortical development.
The protocol serves as a resource for researchers studying neurodevelopmental disorders and NPC homeostasis.