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System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
Published on: April 5, 2017
Therapeutic application of PPE2 protein of Mycobacterium tuberculosis in inhibiting tissue inflammation
Ravi Pal1,2, Madhu Babu Battu1, Sangita Mukhopadhyay1
1Laboratory of Molecular Cell Biology, Center for DNA Fingerprinting and Diagnostics (CDFD), Hyderabad, Telangana, India.
Abstract:
There is an increasing need to develop biological anti-inflammatory agents that are more targeted, effective, and with lesser side effects as compared to conventional chemical drugs. In the present study, we found that Mycobacterium tuberculosis protein PPE2 and a synthetic derivative peptide can suppress the mast cell population and inhibit several vasoactive and fibrogenic mediators and pro-inflammatory cytokines induced by mast cells in formalin-induced tissue injury. PPE2 was found to inhibit transcription from the promoter of stem cell factor, important for mast cell maintenance and migration. Thus, PPE2/peptide can be used as a potent nonsteroidal therapeutic agent for the treatment of inflammation and tissue injury.
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