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Related Experiment Video

Updated: Sep 5, 2025

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining

Published on: June 23, 2020

Multiple rapid-responsive probes for hypochlorite detection based on dioxetane luminophore derivatives.

Yingai Sun¹, Yuqi Gao², Chunchao Tang¹

¹Department of Medicinal Chemistry, Key Laboratory of Chemical Biology (MOE), School of Pharmacy, Shandong University, Jinan, 250012, China.

Journal of Pharmaceutical Analysis

|July 11, 2022

Summary

Researchers developed a novel chemiluminescent probe, HCCL-2, for sensitive detection of hypochlorite. This new tool offers a less invasive and more effective method for tracking hypochlorite in biological studies.

Keywords:

1,2-Dioxetane Chemiluminescent probe Glow-type Hypochlorite

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Area of Science:

Analytical Chemistry
Biochemistry
Biomedical Engineering

Background:

Hypochlorite plays a crucial role as a biomolecule in physiological and pathological processes.
Existing detection methods for hypochlorite are often invasive, costly, and complex.
There is a need for advanced, sensitive, and user-friendly hypochlorite detection tools.

Purpose of the Study:

To develop a novel glow-type chemiluminescent probe for detecting hypochlorite.
To enhance the sensitivity and selectivity of hypochlorite detection for in vitro and in vivo applications.
To create a more accessible tool for studying the functions of hypochlorite.

Main Methods:

Development of a series of chemiluminescent probes (HCCL-1 to HCCL-4) based on Schaap's 1,2-dioxetane luminophores.
Modification of the luminophore scaffold to amplify hypochlorite response signals.
Evaluation of probe performance using the IVIS® imaging system for luminescent intensity, sensitivity, and selectivity.

Main Results:

HCCL-2 demonstrated enhanced sensitivity and selectivity for hypochlorite detection compared to other developed probes.
The limit of detection for HCCL-2 was determined to be 0.166 μM.
The probe successfully tracked hypochlorite both in vitro and in vivo.

Conclusions:

The developed chemiluminescent probe, HCCL-2, offers a sensitive and selective method for hypochlorite detection.
This probe provides a valuable tool for exploring the physiological and pathological roles of hypochlorite.
HCCL-2 represents an advancement over existing methods, offering improved performance for biological research.