Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Sep 5, 2025

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

12.9K

One-Pot Preparation of Collagen Tubes Using Diffusing Gelation.

Yui Iwamoto1, Ryota Haraguchi1, Ryosuke Nakao1

  • 1Department of Chemistry and Applied Chemistry, Saga University, 1 Honjo, Saga 840-8502, Japan.

ACS Omega
|July 11, 2022
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Collagen Xerogel Thread Associated With Reduced Inflammatory and Fibrotic Histological Changes During Early Urethral Wound Healing in Rats.

Cureus·2026
Same author

Intraperitoneal Urine Exposure Does Not Elicit Peritoneal Inflammation or Gastrointestinal Dysmotility: A Histopathological and Functional Study in Rats.

International journal of urology : official journal of the Japanese Urological Association·2026
Same author

UV-Crosslinked Collagen and Gelatin Sheets as Cell Scaffold Materials: Nanoscale Surface Properties and Cell Proliferation Performance.

International journal of molecular sciences·2026
Same author

Development and Characterization of Hollow-Shell Collagen Microcapsules for Three-Dimensional Cell Culture.

Gels (Basel, Switzerland)·2026
Same author

Orally available decitabine prodrug OR-2100 induces mitotic perturbation for the treatment of double-hit lymphoma.

Blood neoplasia·2025
Same author

Sex differences in atrial fibrillation-related atrial remodelling assessed by electroanatomic mapping and biopsy.

European heart journal·2025

Researchers created hollow collagen tubes using a simple one-pot method. Controlling gelation conditions allows for tunable morphology and mechanical strength, suitable for tissue engineering applications.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biotechnology

Background:

  • Collagen is a crucial biopolymer for tissue regeneration.
  • Developing tubular scaffolds with controlled internal architecture is essential for applications like vascular grafts.

Purpose of the Study:

  • To develop a straightforward method for fabricating hollow collagen tubes.
  • To investigate the influence of preparation parameters on the morphology and mechanical properties of these tubes.
  • To assess the potential of these collagen tubes for cell seeding and tissue formation.

Main Methods:

  • One-pot gelation of collagen within capillary glass tubes.
  • Systematic variation of collagen concentration, buffer concentration, and capillary diameter.
  • Morphological analysis of the hollow gel structures.

More Related Videos

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

17.2K
Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

530

Related Experiment Videos

Last Updated: Sep 5, 2025

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells
09:28

Fabrication of Micro-tissues using Modules of Collagen Gel Containing Cells

Published on: December 13, 2010

12.9K
Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo
10:24

Preparation of 3D Collagen Gels and Microchannels for the Study of 3D Interactions In Vivo

Published on: May 9, 2016

17.2K
Production of Nanofibrillar Patterned Collagen for Tissue Engineering
07:34

Production of Nanofibrillar Patterned Collagen for Tissue Engineering

Published on: September 20, 2024

530
  • Mechanical testing (Young's modulus, maximum stress) of dried collagen tubes.
  • In vitro cell seeding with vascular endothelial cells.

    • Main Results:

    • Hollow collagen gels (tubes) with outer diameters under 1 mm were successfully formed.
    • Preparation conditions significantly influenced the cavity's ratio and size, enabling morphological control.
    • Macroscopic phase separation and gelation pinning were identified as mechanisms for hollow formation.
    • Collagen concentration was found to dictate the Young's modulus and maximum stress of the tubes.
    • The material exhibited sufficient mechanical strength for practical applications.
    • Vascular endothelial cells successfully formed layered structures within the gel lumen.

    Conclusions:

    • A facile one-pot method allows for the controlled fabrication of hollow collagen tubes.
    • The mechanical properties are tunable via collagen concentration, meeting requirements for use.
    • These collagen tubes show promise as scaffolds for vascular tissue engineering.