Lab protocol for investigating impacts of climate change on frogs

We developed a method to investigate impacts of temperature (elevated) on breeding, growth and development in endemic frogs under laboratory conditions. The method provides details on housing and rearing of larvae, taking various important measurements and observing developmental deformities. The method could also be applied to rescue approach or head-start program for amphibian species experiencing climate change elsewhere in the world. • Rearing of larvae to investigate effects of temperature on larvae • Measurement of data on growth and development • Rescue/ head-start program.