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Exopolysaccharide Produced by Pediococcus pentosaceus E8: Structure, Bio-Activities, and Its Potential Application
Guangyang Jiang1,2, Juan He3, Longzhan Gan1,2
1College of Biomass Science and Engineering, Sichuan University, Chengdu, China.
Frontiers in Microbiology
|July 11, 2022
Summary
A novel exopolysaccharide, EPS-E8, from Pediococcus pentosaceus E8 exhibits excellent thermal stability and antioxidant properties. This exopolysaccharide shows potential as a food emulsifier and a protective coating for fruit preservation.
Area of Science:
- Microbiology and Biochemistry
- Food Science and Technology
- Materials Science
Background:
- Exopolysaccharides (EPS) produced by lactic acid bacteria are gaining attention for their diverse functional properties.
- Pediococcus pentosaceus E8 secretes a novel exopolysaccharide, designated EPS-E8, with potential applications in food and material science.
Purpose of the Study:
- To characterize the novel exopolysaccharide EPS-E8 produced by Pediococcus pentosaceus E8.
- To evaluate the functional properties of EPS-E8, including its emulsifying, antioxidant, and fruit-coating capabilities.
Main Methods:
- EPS-E8 was isolated and purified using anion-exchange and gel filtration chromatography.
- Structural characterization involved compositional analysis and backbone determination.
- Microstructure was analyzed using atomic force microscopy and scanning electron microscopy.
- Thermal stability, zeta potential, and antioxidant activities (DPPH, ABTS, hydroxyl radical) were assessed.
- Emulsifying properties and efficacy as a strawberry coating were evaluated under various conditions.
Main Results:
- EPS-E8 is a heteropolysaccharide composed of mannose, glucose, and galactose with a molecular weight of 5.02 × 10^4 g/mol.
- Microstructural analysis revealed spherical and reticular shapes, with high thermal stability (305.7°C).
- EPS-E8 demonstrated significant antioxidant capacities and excellent emulsifying properties, stable across different conditions.
- As a coating, EPS-E8 effectively reduced strawberry weight loss and malondialdehyde content while preserving vitamin C and soluble solids.
Conclusions:
- EPS-E8 possesses favorable physicochemical and functional properties, including high thermal stability and antioxidant activity.
- Its robust emulsifying capabilities make it suitable for food applications.
- EPS-E8 shows promise as a polysaccharide-based coating for extending the shelf life of fruits like strawberries.
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