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Updated: Sep 5, 2025

Rapid Fractionation and Isolation of Whole Blood Components in Samples Obtained from a Community-based Setting
Published on: November 30, 2015
How we built a hospital-based community whole blood program.
Joel Addams1, Saman Arbabi2,3, Eileen M Bulger2
1Department of Laboratory Medicine and Pathology, University of Washington School of Medicine, Seattle, Washington, USA.
Implementing a whole blood (WB) program, including low-titer group O WB (LTOWB), can be complex but feasible for prehospital and hospital resuscitation. This initiative successfully integrated LTOWB into trauma care, demonstrating its growing use and potential benefits.
Area of Science:
- Emergency Medicine
- Transfusion Medicine
- Trauma Surgery
Background:
- Whole blood (WB) is a valuable resuscitation product for hemorrhagic shock and massive transfusion protocols.
- Limited experience with WB collection and use necessitated a structured approach for implementation.
- Establishing a WB program requires collaboration between blood banks, hospitals, and emergency medical services.
Purpose of the Study:
- To develop and implement a stepwise process for collecting and utilizing whole blood in clinical practice.
- To integrate low-titer group O RhD positive whole blood (LTOWB) into prehospital and in-hospital resuscitation.
- To assess the feasibility, usage patterns, and initial outcomes of a novel WB program.
Main Methods:
- Collaborated with a regional blood provider to collect cold-stored, leukoreduced, low-titer group O WB.
- Partnered with the local Fire Department to integrate LTOWB into prehospital emergency care.
- Developed comprehensive protocols, software for record-keeping, and training for clinicians and paramedics.
Main Results:
- Between June 2019 and December 2021, 2269 units of LTOWB were received, with 2220 units transfused.
- The majority of transfusions (89%) were administered to trauma patients.
- Monthly usage increased significantly, covering all county fire districts and representing approximately 25% of all hospital trauma blood product use.
Conclusions:
- Developing a WB program is complex but can be initiated gradually, incorporating both prehospital and hospital components.
- Successful implementation requires significant investment in planning, protocols, training, and resources.
- The program has the potential to improve patient care, optimize blood bank and nursing efficiency, and reduce healthcare costs.
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