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Novel human skin surface antimicrobial peptide quantification method using a skin patch test chamber: A pilot study.

Jinyong Lee¹, Oh Sun Kwon¹, Yu Mi Shim¹

¹LG Household & Health Care, LG Science Park, Seoul, Korea.

Journal of Cosmetic Dermatology

|July 11, 2022

Summary

A novel skin patch test offers a noninvasive method to measure antimicrobial peptides (AMPs), specifically human beta-defensins (hBDs), on the skin. This technique minimizes skin damage and shows potential for assessing age-related immune changes.

Keywords:

antimicrobial peptides human beta-defensin innate immunity noninvasive measurement skin

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Area of Science:

Dermatology
Immunology
Biochemistry

Background:

Antimicrobial peptides (AMPs) are crucial for skin's innate immunity against infections.
Current methods like tape stripping (TS) for AMP analysis cause skin damage.
A noninvasive method is needed to assess skin surface AMPs with minimal harm.

Purpose of the Study:

To develop and validate a noninvasive skin patch test for quantifying human beta-defensins (hBDs) on the skin surface.
To compare the efficacy and skin impact of the patch test assay against the traditional TS method.
To investigate age-related differences in skin surface hBD levels and their response to barrier disruption.

Main Methods:

A skin patch test assay was employed to measure hBD concentrations on the skin surface of healthy subjects.
hBD-1 levels were compared between the patch test and TS methods.
Skin physiology and hBD levels were monitored in young and elderly subjects following sodium lauryl sulfate (SLS) induced skin barrier disruption.

Main Results:

The skin patch test demonstrated a significant correlation (Pearson's r=0.640) with the TS method.
The patch test resulted in significantly less skin barrier impairment compared to TS.
Younger subjects exhibited higher skin surface hBD-2 concentrations, particularly after barrier disruption, compared to elderly subjects.

Conclusions:

Skin patch analysis is a convenient and minimally invasive method for evaluating skin surface hBDs.
hBDs can serve as biomarkers for age-related decline in skin's chemical immune response.
This method holds promise for future dermatological and immunological research.