Effects of biodegradable biomedical porous MnO₂ nanoparticles on blood components and functions

In recent years, manganese dioxide (MnO₂) nanoparticles with unique physicochemical properties have been widely used in many biomedical fields, such as biosensors, contrast agents, tumor therapy, and drug delivery. From these applications, MnO₂ nanoparticles have great clinical translation potential. However, by contrast, the in vitro and in vivo biosafety of MnO₂ nanoparticles have been deeply and thoroughly clarified for the clinical translation, which hinders their clinical applications. In this work, we deeply investigated the blood safety of MnO₂ nanoparticles by conducting a series of in vitro and in vivo experiments. These included the effects of MnO₂ nanoparticles on morphology of red blood cells, activation of platelets, coagulation functions, and toxicity of key organs. The obtained results show that these effects displayed a concentration-dependent manner of MnO₂ nanoparticles. Different safe concentration ranges could be found in the different experimental index. This study provides important guidance for the specific biomedical applications of MnO₂ nanoparticles, greatly accelerating their laboratory development and clinical translation.