Related Experiment Video

Updated: Sep 5, 2025

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer
08:12

Detection of a Circulating MicroRNA Custom Panel in Patients with Metastatic Colorectal Cancer

Published on: March 14, 2019

5.6K

miR-129-5p Plays an Anticancer Role in Colon Cancer by Targeting RSF1

Junfeng Hao1, Hongxia Wei2, Yabin Qi1

  • 1Department of General Surgery, Ninth Hospital of Xi 'an, Xi'an, 710054, China. weihongxia6239@163.com.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|July 12, 2022
PubMed

Insights

MicroRNA-129-5p inhibits colon cancer progression by targeting RSF1, impacting cancer cell proliferation and apoptosis. This finding offers a potential therapeutic strategy for colon cancer (CC).

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Colon cancer (CC) remains a significant global health challenge.
  • Understanding the molecular mechanisms underlying CC progression is crucial for developing effective therapies.
  • MicroRNAs (miRNAs) play critical roles in various cellular processes, including cancer development.

Purpose of the Study:

  • To investigate the inhibitory effect of miR-129-5p on colon cancer (CC).
  • To determine if miR-129-5p targets RSF1 in colon cancer cells.
  • To evaluate the impact of miR-129-5p on CC cell proliferation and apoptosis.

Main Methods:

  • Real-time quantitative PCR was employed to analyze miR-129-5p expression in CC and adjacent normal tissues.
  • A retrospective analysis of 56 colon cancer patients was conducted, divided into two groups.
  • Group A (28 patients) received miR-129-5p as a protective factor; Group B (28 patients) received miR-129-5p with an RSF1 carcinoma factor.

Main Results:

  • miR-129-5p expression levels were analyzed in relation to colon cancer proliferation and apoptosis.
  • The study demonstrated that miR-129-5p significantly impacts colon cancer cell proliferation and apoptosis.
  • Targeting RSF1 by miR-129-5p showed a more substantial effect in the experimental group (Group B).

Conclusions:

  • miR-129-5p exhibits a significant inhibitory effect on colon cancer (CC) by targeting RSF1.
  • This microRNA influences colon cancer cell proliferation and apoptosis, suggesting its potential as a therapeutic agent.
  • Targeting the miR-129-5p/RSF1 axis represents a promising avenue for colon cancer treatment strategies.

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

8.5K
Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies09:29

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Published on: September 30, 2016

13.9K

Related Concept Videos

Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
5.3K
Targeted Cancer Therapies02:57

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against...
7.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Ras Gene02:38

The Ras Gene

The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
6.4K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.9K