Adrenergic stimulation of lens cytoskeletal phosphorylation

Stimulation of lens fiber cytoskeletal phosphorylation by adrenergic drugs is described. The effect of isoproterenol on phosphorylation of the 47 Kd beaded filament protein is dose-dependent, detectable as soon as one minute after treatment and blocked by propranolol. Epinephrine increases the phosphorylation of both 47 Kd and the intermediate filament protein, vimentin. 47 Kd phosphorylation is also increased by norepinephrine, dibutyryl-cAMP or forskolin. The results indicate that lens fiber cytoskeletal phosphorylation is regulated, at least in part, via a beta-adrenergic receptor coupled to cyclic AMP production.