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Related Concept Videos

CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

202
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
202

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Related Experiment Video

Updated: Sep 5, 2025

Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9
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Highly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9

Published on: April 10, 2018

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Progress of delivery methods for CRISPR-Cas9.

Wu Yang1,2,3, Jiaqi Yan1,2,3, Pengzhen Zhuang1,2,3

  • 1Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China.

Expert Opinion on Drug Delivery
|July 12, 2022
PubMed
Summary
This summary is machine-generated.

Efficiently delivering CRISPR-Cas9 gene editing tools is crucial for treating diseases. This review explores advanced delivery methods and vectors, overcoming challenges for in vivo applications.

Keywords:
CRISPRCas9drug deliverygene editingreview

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Area of Science:

  • Biotechnology
  • Gene Therapy
  • Nanomedicine

Background:

  • Gene therapy offers potential for treating human diseases.
  • The CRISPR-Cas9 system has revolutionized gene editing.
  • Efficient delivery of CRISPR-Cas9 to target cells remains a major hurdle.

Purpose of the Study:

  • To review the development of CRISPR-Cas9 delivery technologies.
  • To describe various delivery forms and vectors for CRISPR-Cas9.
  • To summarize challenges and strategies in CRISPR-Cas9 delivery.

Main Methods:

  • Review of recent advancements in nanoscience, materials science, and medicine for CRISPR-Cas9 delivery.
  • Analysis of different CRISPR-Cas9 delivery forms (e.g., viral, non-viral vectors).
  • Examination of key barriers and emerging strategies for in vivo and in vitro applications.

Main Results:

  • Various technologies have been developed for CRISPR-Cas9 delivery.
  • Different delivery vectors and forms are emerging for gene editing.
  • Key barriers in delivery systems are being identified and addressed.

Conclusions:

  • CRISPR-Cas9 delivery is critical for clinical applications.
  • Advancements in delivery methods are essential for the future of CRISPR-Cas9 technology.
  • Overcoming delivery challenges will advance in vivo gene editing.