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Relation of beclin-1 and bcl-2 expressions with pathological parameters and prognosis in clear cell renal cell
Rabia Burcin Girgin1, Seyma Ozkanli2, Pinar Engin-Zerk2
1Department of Pathology, Malatya Research and Training Hospital. Turkey.
Introduction:
The most common type of renal cancers is the clear cell renal cell carcinoma (CCRCC) and 98% of CCRCCs have a loss of sequence in the short arm of chromosome 3 by deletion or translocation. Programmed cell death; another possible mechanism of tumorigenesis, comprises two separate components: apoptosis and autophagy. This study aims to show the rela-tion between the prognostic parameters and survival, and Beclin-1, as the representative marker of autophagy, and Bcl-2 as the representative marker of apoptosis in CCRCC patients. In this study, we aimed to determine if Beclin-1 and Bcl-2 expression levels can provide any prognostic information about CCRCC patients.
Methods:
We examined a total of 84 patients who underwent partial or radical nephrectomy and were diagnosed as having CCRCC between January 2008 and December 2015. Immunohistochemical staining was performed, the evaluation was for Beclin-1 and Bcl-2 semi-quantitative, and based on the percentage of positively stained cells (proportion) and staining intensity.
Results:
There was only a statistical significance between Beclin-1 expression and age (r:-0.274; p=0.012; p <0.05). There was a marginal significance between ISUP grade and Beclin-1 (p=0.051). The relation of Bcl-2 expression with the ISUP grade, recurrence, metastasis, and mortality revealed statistical significance (p=0.001, p=0.019, p=0.009, p=0.013, respectively). The ISUP grade and the Bcl-2 expression revealed statistical significance on multivariate analysis ( HR 7.453, 95% CI: 1.935-28.713, p=0.004). The 5-year and 10-year tumor recurrences rates were lower in Bcl-2 positive group, and Bcl-2 positive group experi-enced longer disease free and overall survival.
Conclusion:
There was only marginal correlation between Beclin-1 expression and ISUP grade. No other histopathologic prog-nostic parameters histologic parameters revealed any signigificance. The higher expression of Bcl-2 is correlated with nuclear lower ISUP grade, lower pT stage, and longer disease free and overall survival.
Insights
Clear cell renal cell carcinoma (CCRCC) survival is linked to programmed cell death markers. Higher Bcl-2 (apoptosis marker) expression correlates with better outcomes, while Beclin-1 (autophagy marker) shows limited prognostic value in CCRCC patients.
Area of Science:
- Oncology
- Molecular Biology
- Cancer Research
Background:
- Clear cell renal cell carcinoma (CCRCC) is the most common kidney cancer, often associated with chromosome 3 abnormalities.
- Programmed cell death, encompassing apoptosis and autophagy, plays a role in tumorigenesis.
- Beclin-1 and Bcl-2 are key markers for autophagy and apoptosis, respectively.
Purpose of the Study:
- To investigate the relationship between prognostic parameters and survival in CCRCC patients.
- To determine the prognostic significance of Beclin-1 (autophagy marker) and Bcl-2 (apoptosis marker) expression in CCRCC.
Main Methods:
- Analysis of 84 CCRCC patients diagnosed between 2008 and 2015.
- Immunohistochemical staining to evaluate semi-quantitative expression of Beclin-1 and Bcl-2.
- Assessment based on the percentage of positive cells and staining intensity.
Main Results:
- Beclin-1 expression showed a statistical correlation with patient age and marginal significance with ISUP grade.
- Bcl-2 expression was significantly associated with ISUP grade, recurrence, metastasis, and mortality.
- Higher Bcl-2 expression correlated with lower ISUP grade and pT stage, and predicted longer disease-free and overall survival.
Conclusions:
- Beclin-1 expression has limited prognostic value in CCRCC, with only a marginal correlation to ISUP grade.
- Higher Bcl-2 expression is a significant positive prognostic factor in CCRCC, associated with better survival outcomes.
- Bcl-2 expression serves as a valuable prognostic marker for predicting survival in clear cell renal cell carcinoma.
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