Time-Resolved Luminescent Sensing and Imaging for Enzyme Catalytic Activity Based on Responsive Probes

Area of Science:

• Biochemistry
• Analytical Chemistry
• Biomedical Engineering

Background:

• Enzymes are crucial biomacromolecules in physiological processes and disease development.
• Efficient enzyme activity detection is vital for early disease diagnosis and drug discovery.
• Time-resolved luminescence assays offer high accuracy and signal-to-noise ratios for enzyme detection.

Purpose of the Study:

• To review responsive probes for enzyme activity detection using time-resolved imaging.
• To discuss the design strategies and sensing principles of these probes.
• To highlight current challenges and future prospects in the field.

Main Methods:

• Review of metal complex-based responsive probes.
• Review of metal-free organic compound-based responsive probes.
• Analysis of time-resolved bioimaging and biosensing applications.

Main Results:

• Various responsive probes based on metal complexes and organic compounds have been developed.
• These probes exhibit long luminescence lifetimes, high quantum yields, and photostability.
• Applications in time-resolved imaging for enzyme activity detection are demonstrated.

Conclusions:

• Responsive probes are effective tools for enzyme activity detection via time-resolved imaging.
• Further development is needed to address current challenges and explore future prospects.
• This field holds significant potential for disease diagnosis and drug screening.