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Field and Laboratory Procedures for Fusarium circinatum Identification and Diagnosis
Cristina Zamora-Ballesteros1, Reinaldo Pire2, Julio Javier Diez3
1Sustainable Forest Management Research Institute, University of Valladolid-INIA, Palencia, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|July 12, 2022
Abstract:
Fusarium circinatum is a serious invasive pathogen affecting conifers and causes the disease commonly known as pine pitch canker. Due to the outbreak in European countries, regulations stipulate that Member States must conduct annual official surveys for the fungus on their territory and report the results to the European Commission. Here, we describe the field and laboratory protocols used for the identification and diagnostic of the pathogen.
Keywords:
Agarose gel electrophoresisIdentificationInternal transcribes spacer of the rRNAPCR amplificationPine pitch cankerReal-time PCRSamplingSanger sequencing
