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Updated: Aug 15, 2026

Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 17, 2013
Lysyl-derived aldehydes in outer membrane proteins of Escherichia coli
Abstract:
The major outer membrane proteins from Escherichia coli K-12 are modified to contain alpha-aminoadipic acid delta-semialdehyde (allysine). The allysine was found to be derived from lysine and it was identified by derivatizing it to chloronorleucine by reduction, alpha-aminoadipic acid by oxidation, and to alpha,epsilon-diaminopimelic acid by reacting it with CN- and NH3. The alpha-aminoadipic acid was identified by mass spectrometry. Two major outer membrane proteins were found to possess allysine, a modified lysine characteristically found to connective tissue.
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