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Updated: Sep 5, 2025
Detached Leaf Assays to Simplify Gene Expression Studies in Potato During Infestation by Chewing Insect Manduca sexta
Published on: May 15, 2019
A Detached Leaf Assay for Rapidly Screening Plant Pathogen-Biological Control Agents
Giovana Prado Fortuna Macan1, Sammar Khalil2, Pruthvi B Kalyandurg1
1Department of Plant Breeding, Swedish University of Agricultural Sciences, Alnarp, Sweden.
Abstract:
The detached leaf assay (DLA) is a nondestructive method for evaluating interactions between plants and disease-causing agents that allows quick characterization of potential pathogens' infectivity and plants' resistance to them. Here we show its utility for also assessing potential biological control agents (BCAs), by demonstrating its applicability for screening potential BCAs for the strawberry (Fragaria × ananassa) pathogen Phytophtora cactorum.
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