Pedobacter mucosus sp. nov., isolated from a soil sample of glacier foreland in Austre Lovénbreen, Arctic

A rod-shaped, Gram-stain-negative, non-motile and aerobic bacterium, designated Q8-18^T, was isolated from soil of glacier foreland in Austre Lovénbreen, Arctic, and subjected to a polyphasic taxonomic study. Strain Q8-18^T grew optimally at 20 °C, pH 5.0-8.0 and in the presence of 0-1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q8-18^T belonged to the family Sphingobacteriaceae. Strain Q8-18^T showed the highest sequence similarity to Pedobacter mendelii CCM 8685^T (96.8%), Pedobacter lithocola CCM 8691^T (96.8%), Pedobacter roseus CL-GP80^T (96.7%), Pedobacter changchengzhani E01020^T (96.7%), Pedobacter alluvionis DSM 19624^T (96.6%), Pedobacter jejuensis THG-DR3^T (96.3%), Pedobacter ginsengiterrae DCY49^T (95.9%) and Pedobacter jamesrossensis CCM 8689^T (95.9%). A whole genome-level comparison of strain Q8-18^T with P. roseus CL-GP80^T, P. changchengzhani E01020^T, P. alluvionis DSM 19624^T and Pedobacter heparinus LMG 10339^T revealed average nucleotide identity values of 77.0, 76.0, 77.0 and 70.4%, respectively. The only respiratory isoprenoid quinone was menaquinone-7. The polar lipid profile of strain Q8-18^T was found to contain one phosphatidylethanolamine, eight unidentified aminolipids, one aminophospholipids and five unidentified lipids. The G+C content of the genomic DNA was determined to be 35.4 mol%. The main fatty acids were summed feature 3 (comprising C_16 : 1  ω7c and/or C_16 : 1  ω6c), iso-C_15 : 0 and anteiso-C_15 : 0. On the basis of the evidence presented in this study, a novel species of the genus Pedobacter, Pedobacter mucosus sp. nov., is proposed, with the type strain Q8-18^T (=CCTCC AB 2020009^T=KCTC 82636^T).