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Cancers Originate from Somatic Mutations in a Single Cell

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Related Experiment Video

Updated: Sep 5, 2025

Reusable Single Cell for Iterative Epigenomic Analyses

Reusable Single Cell for Iterative Epigenomic Analyses

Published on: February 11, 2022

Single cell cancer epigenetics.

Marta Casado-Pelaez¹, Alberto Bueno-Costa¹, Manel Esteller²

¹Josep Carreras Leukaemia Research Institute (IJC), Badalona, Barcelona, Catalonia, Spain.

Trends in Cancer

|July 12, 2022

Summary

Single-cell sequencing reveals epigenetic drivers of cancer heterogeneity, overcoming bulk sequencing limitations. This review details methods for studying DNA methylation, chromatin accessibility, and more at the single-cell level to advance cancer research.

Keywords:

cancer epigenetics heterogeneity omics sequencing single cell

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An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

An Integrated Platform for Genome-wide Mapping of Chromatin States Using High-throughput ChIP-sequencing in Tumor Tissues

Published on: April 5, 2018

Area of Science:

Oncology
Genomics
Epigenetics

Background:

Bulk sequencing advanced cancer research but lacks resolution for tumor heterogeneity.
Epigenetic mechanisms are crucial regulators of cancer heterogeneity.
Single-cell sequencing offers a high-resolution approach to study these mechanisms.

Purpose of the Study:

To review single-cell sequencing techniques for epigenetic analysis in cancer.
To explore applications in understanding clonal heterogeneity, tumor microenvironment (TME), and metastasis.
To discuss the limitations of current single-cell epigenetic methodologies.

Main Methods:

Review of single-cell sequencing technologies.
Analysis of epigenetic modifications including DNA methylation, chromatin accessibility, histone modifications, DNA-protein interactions, and chromatin 3D architecture.
Examination of applications in various aspects of cancer heterogeneity.

Main Results:

Single-cell sequencing enables detailed analysis of epigenetic regulation in cancer.
These methods can elucidate clonal heterogeneity, TME dynamics, spatial organization, differentiation, metastasis, and resistance.
Current techniques have technical limitations that require further development.

Conclusions:

Single-cell epigenetics is revolutionizing cancer heterogeneity research.
These advanced techniques provide unprecedented insights into tumor biology.
Addressing technical limitations will further enhance the utility of single-cell epigenetics in oncology.