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Updated: Sep 5, 2025
Optimized PCR-based Detection of Mycoplasma
Published on: June 20, 2011
Detection of Mycoplasma gallisepticum in broiler chickens by PCR
Ebtehal N Mahmmoud1, Mohammad A Hamad1, Zahraa N Khudhur1
1Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, Iraq.
Background:
Mycoplasma is a significant microorganism of poultry, which can cause respiratory infections and synovial inflammation, bringing about huge financial misfortunes to poultry workmanship worldwide.
Aim:
The goal of existing research was to determine the infection rate of Mycoplasma gallisepticum (MG) from chronic respiratory disease cases among broilers fields in Mosul/ Iraq using the polymerase chain reaction (PCR) technique.
Methods:
All 92 lungs samples were collected from broilers with classical respiratory signs in different regions of the Nineveh governorate for 3 months from February to April 2021.
Results:
PCR tests were performed using two couple primers, one for the qualitative amplification of 16S rRNA genes (285 base pairs) in Mycoplasma spp. and the second couple for the detection of M. gallisepticum (580 base pairs). Among the samples obtained from broilers, 87 (94.7%) were positive for Mycoplasma and 79 (85.9%) were positive for M. gallisepticum.
Conclusion:
Our results showed that MG infection in broiler chickens leads to serious clinical symptoms and severe lesions. The rate of Mycoplasma isolation in this study is high despite the short lifespan of broiler chickens.
