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Structural and Functional Changes in Aged Skin Lymphatic Vessels.

Raghu P Kataru1, Hyeung Ju Park1, Jinyeon Shin1

  • 1The Department of Surgery, Division of Plastic and Reconstructive Surgery, Memorial Sloan Kettering Cancer Center, New York, NY, United States.

Frontiers in Aging
|July 13, 2022
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Summary
This summary is machine-generated.

Aging impairs skin lymphatic vessels, reducing fluid and cell transport. This dysfunction exacerbates aging pathology through inflammation and cellular changes, highlighting the lymphatic system's role in aging.

Keywords:
age-related lymphatic dysfunctiondecreased LEC VEGFR-3 signalingdermal lymphaticslymphatic endothelial apoptosisperi-lymphatic inflammation

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Area of Science:

  • Immunology
  • Vascular Biology
  • Aging Research

Background:

  • The lymphatic system is crucial for immune homeostasis and fluid balance.
  • Aging is associated with lymphatic dysfunction, inflammation, and impaired immune responses.
  • Previous research qualitatively assessed aging's impact on lymphatics, but quantitative analysis in skin was lacking.

Purpose of the Study:

  • To quantitatively analyze the effects of aging on skin lymphatic vessels and function.
  • To investigate the cellular mechanisms underlying age-related lymphatic dysfunction.
  • To explore how lymphatic dysfunction contributes to aging pathology.

Main Methods:

  • Fluorescent immunohistochemistry and flow cytometry to assess lymphatic vessel density.
  • Indocyanine green (ICG) lymphangiography and dendritic cell (DC) migration assays to evaluate lymphatic transport.
  • Analysis of VEGFR-3 signaling, LEC apoptosis/senescence, chemokine production, and junctional molecule expression.

Main Results:

  • Aging decreased skin initial and collecting lymphatic vessel density.
  • Aging impaired lymphatic fluid pumping and DC migration.
  • Aging reduced VEGFR-3 signaling, increasing LEC apoptosis and senescence.
  • Aging altered chemokine production and LEC adhesion molecules, promoting inflammation.

Conclusions:

  • Aging significantly impairs skin lymphatic structure and function through multiple cellular mechanisms.
  • Reduced lymphatic function exacerbates aging pathology via increased inflammation and cellular senescence.
  • This study provides quantitative insights into age-related lymphatic dysfunction and its pathological consequences.