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Paediatric pre-B acute lymphoblastic leukaemia-derived exosomes regulate immune function in human T cells
Elham Gholipour1,2,3, Houman Kahroba4,5, Nasim Soltani6
1Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Journal of Cellular and Molecular Medicine
|July 13, 2022
Summary
Leukemia-derived exosomes suppress T cell function and promote apoptosis, shifting immune responses towards a regulatory type. These exosomes may serve as potential liquid biomarkers for cancer staging.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Exosomes from solid tumors impact immune suppression, angiogenesis, and metastasis.
- The role of leukemia-derived exosomes in immune modulation is less understood.
Purpose of the Study:
- To investigate the effects of exosomes from pre-B cell acute lymphoblastic leukemia (ALL) on human T cells.
- To evaluate changes in immune response-related genes and T cell apoptosis.
- To explore the potential of ALL exosomes as biomarkers.
Main Methods:
- Isolation and characterization of exosomes from ALL patient serum.
- Co-incubation of exosomes with T lymphocytes.
- Quantification of gene expression (qRT-PCR) and protein levels (Western blotting, flow cytometry).
Main Results:
- ALL exosomes induced T cell apoptosis and altered T cell profiles towards a regulatory phenotype.
- Increased expression of FOXP3 and Tregs-related cytokines (TGF-β, IL-10).
- Decreased expression of Th17-related factors (RoRγt, IL-17, IL-23).
Conclusions:
- Exosomes from ALL patients possess immunosuppressive properties.
- ALL-derived exosomes may function as liquid biomarkers for cancer staging.
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