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Cell cycle analysis by flow cytometry in an antibody secreting cell line

M Schliermann, C Beckers, H G Miltenburger

Developments in Biological Standardization

|January 1, 1987

Abstract:

This presentation reports on the routine use of flow cytometric techniques in suspension cultures. As a model system an antibody secreting mouse hybridoma cell line was used to monitor antibody production (a Bromodeoxyuridine specific antibody) and proliferative status as obtained by flow cytometry (FCM) and cell counting.

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