Production of human beta-interferon in mouse L-cells

Mouse L-cells, producing glycosilated human beta-interferon constitutively, were cultivated on microcarriers in bioreactors, equipped with a bubble-free aeration system up to 22 1 scale. Suitable aeration membrane surfaces and medium exchange rates permitted cultivation up to 8 X 10(6) cells/ml with a specific beta-interferon productivity of 2 X 10(4) U/10(6) cells/day. A correlation was found between the number of cell layers on the microcarriers and the specific human beta-interferon productivity.