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Updated: Sep 4, 2025

Isolation and Identification of Bacterial Strains from Skin of Terrestrial Amphibians
Published on: June 17, 2025
The intricate molecular identification of Streptomyces: a case study on Antarctic soil isolates
Marcela Proença Borba1, Ana Paula da Silva Ferrero2, Renata de Souza Lameira2
1Programa de Pós-Graduação em Microbiologia Agrícola e do Ambiente, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. ceh.proenca@gmail.com.
Abstract:
Despite the worldwide use of 16S rRNA to identify bacterial species, the use of this gene does not discriminate the 750 species in the genus Streptomyces. A MLST scheme was constructed with rpoB, gyrB, recA, trpB and atpD genes to access the genomic variances in Streptomyces species evolution. We analyze the housekeeping genes in 49 Streptomyces isolates from Antarctic soil. It was used two different databases, GenBank and EzBioCloud to compare the 16S sequences. The species founded in both databases are not the same, but in both cases, a few isolates achieve the necessary high percentage to consider the identification. There is a lack of deposited sequences in the other genes, as the data in GenBank proved to be insufficient. Isolate LMA323St_9 has the potential to be studied as a novel species. Besides that, the use of housekeeping genes gives robust phylogenetic information to understand in group relationships.
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