A protocol to isolate bone marrow innate lymphoid cells for alymphoid mouse reconstitution
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Updated: Sep 4, 2025
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
Published on: July 10, 2013
Nicolas Jacquelot1, Qiutong Huang2, Gabrielle T Belz3
1Princess Margaret Cancer Centre, University Health Network, Toronto, ON M5G 2M9, Canada.
Abstract:
Innate lymphoid cells (ILCs) and adaptive T cells remain a challenge to study because of a significant overlap in their transcriptomic profiles. Here, we describe the adoptive transfer of ILC progenitors into mice genetically deficient in innate and adaptive immune cells to allow detailed study of the development and function of ILCs and gene regulation in an in vivo setting. For complete details on the use and execution of this protocol, please refer to Jacquelot et al. (2021) and Seillet et al. (2016).
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