Stimulation of prostaglandin E production in superfused human amnion by human amniotic fluid

We studied the effects of human amniotic fluid (HAF) on the production of prostaglandin E (PGE) by amnion tissues using a technique of fresh-tissue superfusion. The production of PGE was linear with time for 4.5 h by amnion tissues superfused either with medium alone or with medium plus HAF (30% v/v). Tissues superfused with medium plus HAF produced significantly more PGE than tissues superfused with medium alone. Additionally, amnion tissue obtained at cesarean section, prior to labor, displayed a greater responsiveness to HAF than did amnion obtained after spontaneous labor.