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RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator

Tao Guo1, Ke You2, Xi Chen3

  • 1Department of Pathophysiology, School of Basic Medical Sciences, Weifang Medical University, Weifang, 261053, China.

Cell Death Discovery
|July 13, 2022
PubMed

Insights

RNA-binding motif protein 47 (RBM47) suppresses hepatocellular carcinoma (HCC) growth and metastasis. RBM47 upregulates the tumor suppressor UPF1 at both transcriptional and posttranscriptional levels, highlighting its role in HCC progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Hepatocellular carcinoma (HCC) progression mechanisms, including growth and metastasis, are poorly understood.
  • Molecular dysregulation significantly impacts cellular signaling pathways in HCC.
  • The role of RNA-binding motif protein 47 (RBM47) as a tumor regulator in HCC is currently ambiguous.

Purpose of the Study:

  • To investigate the function of RBM47 in hepatocellular carcinoma (HCC).
  • To elucidate the molecular mechanisms by which RBM47 influences HCC tumor behaviors.
  • To determine if RBM47 acts as a tumor suppressor or promoter in HCC.

Main Methods:

  • In vitro assays to assess RBM47's influence on tumor behaviors.
  • In vivo xenograft models to evaluate RBM47's effect on tumor growth and metastasis.
  • Analysis of RBM47's interaction with UPF1 mRNA and its transcriptional regulation.

Main Results:

  • RBM47 demonstrated an inhibitory effect on HCC tumor behaviors in vitro.
  • RBM47 suppressed xenograft tumor growth and metastasis in vivo.
  • RBM47 was found to positively regulate Upframeshift 1 (UPF1) expression.
  • RBM47 enhances UPF1 mRNA stability by binding to its 3'UTR and promotes UPF1 transcription.

Conclusions:

  • RBM47 functions as a tumor suppressor in hepatocellular carcinoma (HCC).
  • RBM47 exerts its tumor-suppressive role by upregulating UPF1.
  • RBM47 acts as a DNA/RNA binding protein, regulating UPF1 at both transcriptional and posttranscriptional levels.

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