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Updated: Sep 4, 2025
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Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis
Published on: June 27, 2020
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A functional reference map of the RNF8 interactome in cancer
Chuanyang Liu1, Jingyu Kuang2, Yuxuan Wang1
1Department of Biology and Chemistry, College of Sciences, National University of Defense Technology, Changsha, 410073, Hunan, China.
Biology Direct
|July 13, 2022
Summary
Researchers mapped the RNF8 interactome, revealing new targets and functions. This work advances understanding of RNF8 in cancer and neurodegenerative diseases, aiding anti-RNF8 therapy development.
Area of Science:
- Molecular Biology
- Genomics
- Proteomics
Background:
- RNF8 is a DNA damage-responsive E3 ligase and a potential cancer therapeutic target.
- Limited understanding of RNF8's interactome and diverse cancer roles hinders therapeutic development.
- High-throughput methods are needed to map the RNF8 interactome.
Purpose of the Study:
- To identify and analyze the RNF8 interactome using high-throughput methods.
- To uncover novel RNF8 biological functions and gene-disease associations.
- To explore RNF8's role in cancer and neurodegenerative diseases.
Main Methods:
- A two-way liquid chromatography-mass spectrometry (LC-MS) approach was employed for RNF8 interactome identification.
- In silico analysis and in vitro validation were used to confirm interactome components.
- Bulk and single-cell RNA sequencing (scRNA-seq) datasets were analyzed to assess gene-disease associations.
Main Results:
- A comprehensive RNF8 interactome map was generated, identifying new targets like YBX1, DNMT1, and HDCA1.
- RNF8 was linked to neurodegenerative diseases and tumor-infiltrating immune cells.
- Direct binding and ubiquitination of YBX1 by RNF8 were validated, highlighting RNF8 as a YBX1 regulator.
Conclusions:
- The study provides a framework for understanding RNF8-regulated functions in cancer.
- The findings facilitate the rational design and development of anti-RNF8 cancer therapies.
- RNF8's role in neurodegenerative diseases and immune cell infiltration warrants further investigation.
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