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Related Experiment Video

Updated: Sep 4, 2025

Sequence-specific and Selective Recognition of Double-stranded RNAs over Single-stranded RNAs by Chemically Modified Peptide Nucleic Acids
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Charge-Conversion Strategies for Nucleic Acid Delivery.

Kingshuk Dutta1,2, Ritam Das1,3, Jewel Medeiros1,3

  • 1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, United States.

Advanced Functional Materials
|July 14, 2022
PubMed
Summary
This summary is machine-generated.

Charge-converting nanocarriers offer innovative solutions for nucleic acid therapeutics delivery. These advanced carriers overcome limitations like cytotoxicity and poor endosomal escape, improving gene therapy and vaccine development.

Keywords:
Charge ConversionDrug DeliveryEndosomal EscapeNon-cationicNucleic Acid

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Area of Science:

  • Biotechnology
  • Nanomedicine
  • Molecular Biology

Background:

  • Nucleic acids are potent therapeutic agents with applications in gene therapy, gene editing, and vaccine development.
  • Current nucleic acid drug delivery faces challenges, including cationic charge-induced cytotoxicity and inefficient delivery.
  • Traditional carriers exhibit limitations such as serum deactivation, instability, low transfection rates, and poor endosomal escape.

Purpose of the Study:

  • To critically analyze molecular designs of charge-converting nanocarriers for nucleic acid delivery.
  • To explore innovative carrier strategies that overcome critical barriers in therapeutic delivery.
  • To discuss the clinical translation and future scope of charge-converting nanocarrier approaches.

Main Methods:

  • Review and classification of various molecular designs for charge-converting nanocarriers.
  • Analysis of charge-conversion mechanisms (cationic to neutral/anionic, anionic to cationic).
  • Evaluation of carrier performance in overcoming delivery barriers like serum stability and endosomal escape.

Main Results:

  • Charge-converting nanocarriers demonstrate potential to mitigate cytotoxicity associated with cationic charges.
  • These carriers enhance stability, improve transfection efficiency, and facilitate endosomal escape.
  • Various charge-conversion strategies have been developed to address specific delivery challenges.

Conclusions:

  • Charge-converting nanocarriers represent a promising advancement for effective nucleic acid therapeutics delivery.
  • These innovative designs address key limitations of traditional carriers, paving the way for improved molecular medicines.
  • Further research and clinical evaluation are essential to realize the full potential of these approaches.