Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing patient-specific computational modeling of cardiac mechanics by leveraging high spatiotemporal resolution magnetic resonance imaging.

Computer methods and programs in biomedicine·2026
Same author

Characterization of Lysosomal Hydrolases and Transporters and Their Age-Dependent Variability: Relevance to Drug Metabolism and Transport of Small Molecule and Biologic Drugs.

Clinical pharmacology and therapeutics·2026
Same author

Temporal trends in anal HPV infection among men who have sex with men attending sexual health clinics in three United States cities, 2018-2024.

Sexually transmitted diseases·2026
Same author

Cost-effectiveness of integrated prevention for late-life depressive symptoms: Real-world findings from a large-scale pragmatic trial ("JoyAge").

Journal of affective disorders·2026
Same author

Recent Advances in Nanoparticle-Based Drug Delivery Strategies to Cross the Blood-Brain Barrier in Targeted Treatment of Alzheimer's Disease.

Pharmaceutics·2026
Same author

Perioperative outcomes of laparoscopic donor nephrectomy: A single-center experience.

Urology annals·2026

Related Experiment Video

Updated: Sep 4, 2025

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method
06:13

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method

Published on: March 31, 2022

3.7K

18O-Enabled High-Throughput Acyl Glucuronide Stability Assay.

Zaikuan Josh Yu1, Hoa Le1, Jennifer Tang1

  • 1Drug Metabolism, Gilead Sciences Inc., Foster City, California 94404, United States.

Chemical Research in Toxicology
|July 14, 2022
PubMed
Summary
This summary is machine-generated.

A new 18O-labeling assay simplifies acyl glucuronide (AG) stability assessment. This method accurately predicts AG migration tendency, crucial for evaluating drug toxicity risks early in discovery.

More Related Videos

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

4.0K

Related Experiment Videos

Last Updated: Sep 4, 2025

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method
06:13

Determination of Glucan Chain Length Distribution of Glycogen Using the Fluorophore-Assisted Carbohydrate Electrophoresis FACE Method

Published on: March 31, 2022

3.7K
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
12:02

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

11.6K
Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
12:19

Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

Published on: April 22, 2022

4.0K

Area of Science:

  • Biochemistry
  • Pharmacology
  • Drug Metabolism

Background:

  • Acyl glucuronides (AGs) are metabolites of carboxylic acid drugs.
  • AGs can form covalent adducts, potentially leading to drug toxicity.
  • Assessing AG chemical stability is vital for drug safety.

Purpose of the Study:

  • To develop a high-throughput assay for acyl glucuronide stability.
  • To eliminate the need for liquid chromatography (LC) method development.
  • To facilitate early-stage drug liability assessment.

Main Methods:

  • Utilized 18O-incorporation from [18O] water to monitor AG stability.
  • Compared 18O-labeling rates with AG migration tendencies.
  • Explored in situ AG generation using human hepatic microsomes.

Main Results:

  • Faster 18O-incorporation correlated with shorter AG migration half-lives.
  • Differential 18O-incorporation levels accurately predicted AG migration tendency.
  • In situ generated AGs yielded results comparable to authentic standards.

Conclusions:

  • The 18O-labeling assay provides a simplified, high-throughput method for AG stability assessment.
  • This assay requires no LC method development or AG reference standards.
  • It effectively facilitates early drug discovery by assessing acyl glucuronide liability.