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Updated: Sep 4, 2025
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Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
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Microbiota in sports.
Katarzyna Mańkowska1, Małgorzata Marchelek-Myśliwiec2, Piotr Kochan3
1Department of Laboratory Medicine, Chair of Microbiology, Immunological Diagnostics and Laboratory Medicine, Pomeranian Medical University, Al. Powstańców Wlkp 72, 70-110, Szczecin, Poland. katarzyna.mankowska@pum.edu.pl.
Archives of Microbiology
|July 14, 2022
Summary
Physical activity and diet significantly impact athlete gut microbiota composition. Specific bacteria increase with exercise, and fiber-rich diets promote beneficial microbes, suggesting probiotic supplementation may enhance performance.
Area of Science:
- Microbiology
- Sports Science
- Human Physiology
Background:
- The human gut microbiota plays a crucial role in health and disease.
- Microbiota composition is influenced by genetics, lifestyle, diet, and physical activity.
- Understanding athlete microbiota is key to optimizing performance and health.
Purpose of the Study:
- To analyze the relationship between physical activity, diet, and gastrointestinal microbiota in athletes.
- To review differences in microbial composition across sports disciplines.
- To explore the impact of probiotics on athlete microbiome and performance.
Main Methods:
- Literature review analyzing existing studies on athlete microbiota.
- Examination of factors influencing gut bacteria diversity in athletes.
- Correlation analysis between diet, exercise, and specific microbial genera.
Main Results:
- Physical activity increases specific bacteria (e.g., Lactobacillus, Prevotella, Bacteroides, Veillonella), varying by sport.
- High VO2 max sports correlate with higher abundance of certain beneficial bacteria.
- A diet rich in dietary fiber promotes Lactobacillus and Bifidobacterium growth.
Conclusions:
- Athlete gut microbiota composition is dynamic and influenced by sport type and diet.
- Probiotic supplementation may be beneficial for athletes, potentially improving sports results.
- Further research is needed to fully elucidate microbiota changes in athletes.
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