Full Assessment of Lung Mechanics Using Computer-Controlled, Forced Oscillation Technique

Area of Science:

• Physiology
• Respiratory Medicine
• Animal Models

Background:

• Accurate quantification of respiratory system mechanical properties is crucial for understanding lung diseases.
• The forced oscillation technique (FOT) offers a powerful method for assessing airway and tissue mechanics.
• Standardized protocols are needed for reliable lung function phenotyping in preclinical research.

Purpose of the Study:

• To provide a detailed, standardized protocol for measuring mouse respiratory mechanical parameters using FOT.
• To adapt and implement FOT for lung-function phenotyping of mouse models within the International Mouse Phenotyping Consortium (IMPC).
• To offer a practical guide for researchers interested in applying FOT for lung function evaluation.

Main Methods:

• Detailed protocol for mouse endotracheal intubation using a custom-built platform and confirmation module.
• Utilization of the FlexiVentFX™ system for comprehensive lung function assessment.
• Application of FlexiWare™ software for experimental planning, execution, and data analysis.

Main Results:

• A standardized and adaptable protocol for mouse lung function assessment using FOT has been established.
• The protocol integrates simplified steps, technical considerations, and hardware-software demonstration.
• The method is suitable for lung-function phenotyping of mouse models, including those for the IMPC.

Conclusions:

• This protocol provides a robust and implementable method for evaluating mouse respiratory mechanics via FOT.
• The detailed procedure and integrated system facilitate accurate lung function phenotyping.
• The protocol is adaptable for researchers seeking to utilize FOT in their lung research.