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Updated: Sep 4, 2025
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
Published on: May 8, 2013
A fusidic acid-resistant (PVL+) clone is associated with the increase in methicillin-resistant Staphylococcus aureus
Alexandre Bourles1, Anne Tristan2, François Vandenesch2
1Institut Pasteur de Nouvelle-Calédonie, Institut Pasteur International Network, Medical and Environmental Bacteriology Group - Bacteriology Unit, Noumea, New Caledonia.
Objectives:
Since 2014, Staphylococcus aureus methicillin resistance has been rapidly increasing in New Caledonia and is associated with potential serious clinical repercussions. In the present study, we investigated the epidemiology of methicillin-resistant S. aureus (MRSA) in New Caledonia and the possible emergence of a particular clonal strain.
Methods:
An overview of the distribution of MRSA in New Caledonia in 2019 is presented. We collected and analysed 171 clinical MRSA isolates from New Caledonia medical laboratories during August and September 2019. Among this collection, 49 representative isolates were analyzed by the French National Reference Center for Staphylococci using the StaphyType DNA microarray, allowing genetic characterization of the isolates.
Results:
Among the 1144 S. aureus isolated over the year 2019, 442 isolates (39%) were resistant to methicillin, and 62% of these isolates were resistant to fusidic acid (FA). During the inclusion period, FA resistance rate was similar (60%). Genetic characterization evidenced CC6 as the predominant clonal complex (70%) with 26 isolates (53%) identified as CC6-MRSA-[IV+fus] (PVL+).
Conclusions:
These findings demonstrated a low diversity of MRSA in New Caledonia, with the dominance of a clonal complex not reported previously. The frequent fusidic acid (FA) resistance in MRSA was associated with a high prevalence of fusC gene, suggesting that FA misuse contributed to driving the selection of this clone. Our findings suggest the recommendation to stop the topical use of FA to control the emergence of this severe MRSA clone and decrease the rate of MRSA in New Caledonia.
Insights
Methicillin-resistant Staphylococcus aureus (MRSA) is increasing in New Caledonia. A dominant clone (CC6-MRSA) with high fusidic acid resistance was identified, suggesting topical fusidic acid misuse drives MRSA emergence.
Area of Science:
- Microbiology
- Epidemiology
- Genetics
Background:
- Methicillin-resistant Staphylococcus aureus (MRSA) has shown a rapid increase in New Caledonia since 2014.
- This rise in MRSA is linked to significant clinical complications.
Purpose of the Study:
- To investigate the epidemiology of MRSA in New Caledonia.
- To identify the potential emergence of a specific clonal strain.
Main Methods:
- Analysis of 171 clinical MRSA isolates from New Caledonia in 2019.
- Genetic characterization of 49 representative isolates using StaphyType DNA microarray by the French National Reference Center for Staphylococci.
Main Results:
- In 2019, 39% of Staphylococcus aureus isolates were MRSA (442/1144).
- 62% of MRSA isolates exhibited resistance to fusidic acid (FA).
- Clonal complex 6 (CC6) was predominant (70%), with CC6-MRSA-[IV+fus] (PVL+) identified in 53% of these.
Conclusions:
- Low MRSA diversity was observed, dominated by a previously unreported clonal complex.
- High FA resistance correlated with the fusC gene, suggesting FA misuse as a selection driver.
- Recommendations include halting topical FA use to control this MRSA clone and reduce overall MRSA rates.
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